Biodistribution and lymph node retention of polysaccharide-based immunostimulating nanocapsules.
Identifieur interne : 000237 ( Main/Exploration ); précédent : 000236; suivant : 000238Biodistribution and lymph node retention of polysaccharide-based immunostimulating nanocapsules.
Auteurs : RBID : pubmed:24508040Abstract
The adjuvant properties of polyglucosamine/squalene-based nanocapsules (PG-nanocapsules) associated with different subunit antigens has been previously reported. Thus, the aim of the present study was to monitor the biodistribution of PG-nanocapsules and their affinity for the draining lymph nodes after subcutaneous (s.c.) injection. The nanocapsules were efficiently radiolabeled with indium-111 ((111)In) (labeling efficiency of 98%). The diameter and zeta potential values of the unlabeled nanocapsules was preserved after the radiolabeling process and only 20% of the (111)In dissociated from the nanocapsules after 48h of incubation in serum. The radiolabeled nanocapsules and the control (111)InCl3 in saline solution (18.5MBq (500μCi) in 100μL) were injected s.c. in New Zealand White rabbits. The γ-scintigraphy imaging analysis revealed a slow clearance of the nanocapsules from the injection site and their progressive accumulation in the popliteal lymph node over time (3.8%±1.2 of the injected dose at 48h). Indeed, the clearance rate of the nanocapsules from the injection site was significantly slower than that of the control (free (111)InCl3), which rapidly drained into systemic circulation and accumulated mainly in excretion organs (i.e. kidneys and liver). In contrast, the biodistribution of nanocapsules was preferably limited to the lymphatic circulation. These results suggest that the immune potentiating effect previously observed for PG-nanocapsules is mainly due to the formation of a depot at the injection site, which was followed by a slow drainage into the lymphatic system and a prolonged retention in the lymph nodes.
DOI: 10.1016/j.vaccine.2014.01.059
PubMed: 24508040
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biodistribution and lymph node retention of polysaccharide-based immunostimulating nanocapsules.</title><author><name sortKey="Vicente, Sara" uniqKey="Vicente S">Sara Vicente</name><affiliation wicri:level="1"><nlm:affiliation>Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela</wicri:regionArea></affiliation></author><author><name sortKey="Goins, Beth A" uniqKey="Goins B">Beth A Goins</name><affiliation wicri:level="1"><nlm:affiliation>Radiology Department, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Radiology Department, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900</wicri:regionArea></affiliation></author><author><name sortKey="Sanchez, Alejandro" uniqKey="Sanchez A">Alejandro Sanchez</name><affiliation wicri:level="1"><nlm:affiliation>Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela 15706, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela 15706</wicri:regionArea></affiliation></author><author><name sortKey="Alonso, Mar A J" uniqKey="Alonso M">María J Alonso</name><affiliation wicri:level="1"><nlm:affiliation>Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela 15706, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela 15706</wicri:regionArea></affiliation></author><author><name sortKey="Phillips, William T" uniqKey="Phillips W">William T Phillips</name><affiliation wicri:level="1"><nlm:affiliation>Radiology Department, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA. Electronic address: Phillips@uthscsa.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Radiology Department, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><date when="2014">2014</date><idno type="RBID">pubmed:24508040</idno><idno type="pmid">24508040</idno><idno type="doi">10.1016/j.vaccine.2014.01.059</idno><idno type="wicri:Area/Main/Corpus">000175</idno><idno type="wicri:Area/Main/Curation">000175</idno><idno type="wicri:Area/Main/Exploration">000237</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The adjuvant properties of polyglucosamine/squalene-based nanocapsules (PG-nanocapsules) associated with different subunit antigens has been previously reported. Thus, the aim of the present study was to monitor the biodistribution of PG-nanocapsules and their affinity for the draining lymph nodes after subcutaneous (s.c.) injection. The nanocapsules were efficiently radiolabeled with indium-111 ((111)In) (labeling efficiency of 98%). The diameter and zeta potential values of the unlabeled nanocapsules was preserved after the radiolabeling process and only 20% of the (111)In dissociated from the nanocapsules after 48h of incubation in serum. The radiolabeled nanocapsules and the control (111)InCl3 in saline solution (18.5MBq (500μCi) in 100μL) were injected s.c. in New Zealand White rabbits. The γ-scintigraphy imaging analysis revealed a slow clearance of the nanocapsules from the injection site and their progressive accumulation in the popliteal lymph node over time (3.8%±1.2 of the injected dose at 48h). Indeed, the clearance rate of the nanocapsules from the injection site was significantly slower than that of the control (free (111)InCl3), which rapidly drained into systemic circulation and accumulated mainly in excretion organs (i.e. kidneys and liver). In contrast, the biodistribution of nanocapsules was preferably limited to the lymphatic circulation. These results suggest that the immune potentiating effect previously observed for PG-nanocapsules is mainly due to the formation of a depot at the injection site, which was followed by a slow drainage into the lymphatic system and a prolonged retention in the lymph nodes.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">24508040</PMID><DateCreated><Year>2014</Year><Month>03</Month><Day>18</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2518</ISSN><JournalIssue CitedMedium="Internet"><Volume>32</Volume><Issue>15</Issue><PubDate><Year>2014</Year><Month>Mar</Month><Day>26</Day></PubDate></JournalIssue><Title>Vaccine</Title><ISOAbbreviation>Vaccine</ISOAbbreviation></Journal><ArticleTitle>Biodistribution and lymph node retention of polysaccharide-based immunostimulating nanocapsules.</ArticleTitle><Pagination><MedlinePgn>1685-92</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.vaccine.2014.01.059</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0264-410X(14)00111-X</ELocationID><Abstract><AbstractText>The adjuvant properties of polyglucosamine/squalene-based nanocapsules (PG-nanocapsules) associated with different subunit antigens has been previously reported. Thus, the aim of the present study was to monitor the biodistribution of PG-nanocapsules and their affinity for the draining lymph nodes after subcutaneous (s.c.) injection. The nanocapsules were efficiently radiolabeled with indium-111 ((111)In) (labeling efficiency of 98%). The diameter and zeta potential values of the unlabeled nanocapsules was preserved after the radiolabeling process and only 20% of the (111)In dissociated from the nanocapsules after 48h of incubation in serum. The radiolabeled nanocapsules and the control (111)InCl3 in saline solution (18.5MBq (500μCi) in 100μL) were injected s.c. in New Zealand White rabbits. The γ-scintigraphy imaging analysis revealed a slow clearance of the nanocapsules from the injection site and their progressive accumulation in the popliteal lymph node over time (3.8%±1.2 of the injected dose at 48h). Indeed, the clearance rate of the nanocapsules from the injection site was significantly slower than that of the control (free (111)InCl3), which rapidly drained into systemic circulation and accumulated mainly in excretion organs (i.e. kidneys and liver). In contrast, the biodistribution of nanocapsules was preferably limited to the lymphatic circulation. These results suggest that the immune potentiating effect previously observed for PG-nanocapsules is mainly due to the formation of a depot at the injection site, which was followed by a slow drainage into the lymphatic system and a prolonged retention in the lymph nodes.</AbstractText><CopyrightInformation>Published by Elsevier Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vicente</LastName><ForeName>Sara</ForeName><Initials>S</Initials><Affiliation>Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain.</Affiliation></Author><Author ValidYN="Y"><LastName>Goins</LastName><ForeName>Beth A</ForeName><Initials>BA</Initials><Affiliation>Radiology Department, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA.</Affiliation></Author><Author ValidYN="Y"><LastName>Sanchez</LastName><ForeName>Alejandro</ForeName><Initials>A</Initials><Affiliation>Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela 15706, Spain.</Affiliation></Author><Author ValidYN="Y"><LastName>Alonso</LastName><ForeName>María J</ForeName><Initials>MJ</Initials><Affiliation>Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Pharmacy and Pharmaceutical Technology Department, School of Pharmacy, University of Santiago de Compostela, 15705 Campus Vida, Santiago de Compostela, Spain; Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela 15706, Spain.</Affiliation></Author><Author ValidYN="Y"><LastName>Phillips</LastName><ForeName>William T</ForeName><Initials>WT</Initials><Affiliation>Radiology Department, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, USA. Electronic address: Phillips@uthscsa.edu.</Affiliation></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>02</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Vaccine</MedlineTA><NlmUniqueID>8406899</NlmUniqueID><ISSNLinking>0264-410X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biodistribution</Keyword><Keyword MajorTopicYN="N">Chitosan</Keyword><Keyword MajorTopicYN="N">Imaging</Keyword><Keyword MajorTopicYN="N">Lymph node</Keyword><Keyword MajorTopicYN="N">Nanocarrier</Keyword><Keyword MajorTopicYN="N">Nanovaccine</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>8</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>1</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>1</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2014</Year><Month>2</Month><Day>7</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>2</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24508040</ArticleId><ArticleId IdType="pii">S0264-410X(14)00111-X</ArticleId><ArticleId IdType="doi">10.1016/j.vaccine.2014.01.059</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=IndiumV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000237 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000237 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= *** parameter Area/wikiCode missing ***
|area= IndiumV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:24508040
|texte= Biodistribution and lymph node retention of polysaccharide-based immunostimulating nanocapsules.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24508040" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a IndiumV2
| This area was generated with Dilib version V0.5.76. |  |